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| Registros recuperados: 67 | |
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| Tsai,S.; Lin,C.. |
| Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014 |
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| Morais,Hévea de; Spuri,Renata; Gonçalves,Tarcísio de Moraes; Carvalho,Rafaela Rodrigues de; Andrade,Renato Campos; Pinto,Tássia Louregiani Carvalho; Souza,José Camisão de. |
| The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimulation (6 Holstein and 8 Tabapuã), 500 mg of rbST (Boostin®) on D0 and 500 IU of eCG (Folligon) on D2; and control (6 Holstein and 8 Tabapuã), in which heifers received injections of the excipient. Heifers were aspirated on D4. Oocytes were subjected to a well established commercial in vitro embryo production protocol (Vitrogen®) and embryos were evaluated seven days after fertilization. There was an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Hormonal stimulation; Ovary. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200004 |
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| Oliveira,F.D.; Sangalli,J.R.; Meirelles,F.V.; Perecin,F.; Silva Filho,P.P.C.; Watanabe,Y.F.; Miglino,M.A.; Assis Neto,A.C.. |
| In cattle, embryo development is characterized by the appearance of two distinct cell layers, the trophectoderm and the inner cell mass. The latter will undergo differentiation to form the embryonic disc consisting of the epiblast and hypoblast. The aim of this study was to ultrastructurally characterize the bovine embryo from different in vitro production techniques, with emphasis on trophectoderm and inner cell mass cells. Bovine embryos on day 7 (conception = D1) of pregnancy, derived via in vitro production techniques, were fixed for light and transmission electron microscopy processing. Results suggested that embryos produced by nuclear transfer of somatic cells and parthenogenesis showed significant changes in macroscopic and microscopic structure.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Blastocyst; In vitro production; Ultrastructure. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000601483 |
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| Talukder, Anup K.; Yousef, Mohamed S.; Rashid, Mohammad B.; Awai, Kensuke; Acosta, Tomas J.; Shimizu, Takashi; Okuda, Kiyoshi; Shimada, Masayuki; Imakawa, Kazuhiko; Miyamoto, Akio. |
| Recent observations suggest that the bovine uterus starts to react to the early embryo immediately after its arrival from the oviduct. The present study aimed to investigate the effect of the early developing embryo on the immune-related gene profile in bovine uterine epithelial cells (BUECs) in vitro, and to further examine the impact of conditioned media (CM), either from embryo-BUEC co-culture or embryo culture alone, on gene expression in peripheral blood mononuclear cells (PBMCs). First, BUECs were co-cultured with morulae (n = 10) for D5-D9 (D0 = IVF), and gene expression in BUECs was analyzed. Subsequently, PBMCs were cultured in CM from embryo-BUEC co-culture or D5-D9 embryo culture, and gene expression was evaluated. In BUECs, the embryo induced... |
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Palavras-chave: Bovine; Embryo; Immunity; Interferon tau (IFNT); Uterus. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4532 |
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| OKAWARA, Sumika; HAMANO, Seizo; TETSUKA, Masafumi; 手塚, 雅文. |
| Glycerol plays multi-functional roles in cellular physiology. Other than forming the backbone molecule for glycerophospholipid and triglyceride (TG), glycerol acts as an energy substrate for glycolysis. Spermatozoa are known to utilize glycerol for energy production, but there are no reports of this in oocytes. In this study, the value of glycerol as an energy substrate for bovine oocyte maturation (Exp. 1) and the gene expression of glycerol kinase (GK), an enzyme crucial for cellular glycerol utilization, in bovine oocytes and early embryos (Exp. 2) were examined. In Exp. 1, in vitro maturation (IVM) was conducted using synthetic oviduct fluid supplemented with/without glucose (1.5 mM) and/or glycerol (1.0 mM), and maturation rate, degree of cumulus... |
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Palavras-chave: Bovine; Embryo; Glycerol; Glycerol kinase (GK); Oocyte maturation. |
| Ano: 2009 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2829 |
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| IOSSI,EMERSON; MORO,FABIOLA VITTI; VIEIRA,BRUNO GUILHERME TORRES LICURSI; BARBOSA,RAFAEL MARANI; VIEIRA,ROBERVAL DAITON. |
| ABSTRACT Queen palm is a tropical species of ecological importance and economic potential in various uses, including food. However, the knowledge about the nutritional value of fruits and the estimation of seed viability are still scarce. The aims of this work were to study the biometrics and chemical composition of fruits and seeds; describe the anatomy of embryos and assess the viability of the tetrazolium test. Mature fruits were collected, processed and submitted to biometry, water content, pulp and seeds chemical composition, germination and emergence in the laboratory evaluations. For the tetrazolium test, whole embryos were immersed in solution at 40 °C for one, two, four and six hours and compared with germination. The fruit pulp is rich in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Queen palm; Embryo; Germination; Palm; Pyrene. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000402001 |
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| Fakheri,Taravat; Nankali,Anisodowleh; Khazaei,Mozafar; Ghanbari,Ali; Abedini,Mehrandokht; Saeidiborojeni,Hamidreza. |
| There were no significant differences in the distribution of embryos reaching to 2- cells, 4- cells, morula or blastocysts culturing on human endometrial stromal cells (Secretory or proliferative phases). The percent of morula in stage A (without fragmentation), stage B (<25% fragmentation), stage C (25-50% fragmentation) and stage D (>50% fragmentation) and did not showed significant differences between two coculture groups. Thus, the phase that the endometrial stromal cells were in thereby did not affect on the quality of embryos. |
| Tipo: Journal article |
Palavras-chave: Co-culture; Embryo; Endometrium; Mice; Proliferative phase; Secretory phase. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000400014 |
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| Onbaşilar,EE; Erdoğan,M; Erol,S; Kahraman,M; Güngör,ÖF. |
| ABSTRACT Pure breeds are important due to the fact that they adapt well to poor husbandry conditions. However, gene expression of digestive enzymes and nutrient transporters of pure breeds and layer hybrids have not been studied yet. Therefore, this study was directed to detect the gene expression of digestive enzymes and nutrient transporters in the yolk sac membrane and small intestine of the embryos in layer hybrid and pure breeds. A total of 354 hatching eggs were collected from 29 wk old Denizli, Gerze and Lohman White (LW) breeder flocks. Twelve eggs from each genotype were selected at embryonic day 19 (E19) and day 21 (E21). APN, SI, PepT1, EAAT3, CAT1, B0AT, SGLT1 and GLUT5 mRNA expressions were evaluated in the yolk sac membrane (YSM) and small... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Gene expression; Layer hybrid; Pure breed. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000300435 |
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| Mellado Vázquez, Adriana. |
| Se estudió la influencia de las condiciones de cultivo (clima, riego o temporal y tipo de suelo) en la composición nutrimental de los tejidos del fruto (epicarpio, pulpa, testa y embrión) y en la remoción de nutrimentos por tejido y por tonelada de fruto fresco de los cvs. Ataulfo, Kent, Keitt y Tommy Atkins. Asimismo, en ‘Ataulfo’ y ‘Tommy Atkins’ se estudió la relación entre el contenido nutrimental en suelo, hojas, epicarpio y pulpa (materia seca) y pulpa fresca, con la calidad postcosecha. Se trabajó con árboles altamente productivos (producción superior a los 150 kg·árbol-1), seleccionados en el 2008 de los que se cosecharon frutos en madurez fisiológica. En la materia seca de cada tejido se determinó la concentración de: N, P, K, Ca, Mg, S, Fe, Cu,... |
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Palavras-chave: Mangifera indica; Epicarpio; Pulpa; Testa; Embrión; Calidad postcosecha; Skin; Pulp; Seed coat; Embryo; Post-harvest quality; Doctorado; Fruticultura. |
| Ano: 2012 |
URL: http://hdl.handle.net/10521/736 |
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| Lacoue Labarthe, T; Warnau, A; Oberhansli, F; Teyssie, J; Koueta, Noussithe; Bustamante, Paco. |
| Cuttlefish eggs were exposed to background concentrations of dissolved Ag and Cd, using the radiotracers Ag-110m and Cd-109. At different time of the embryonic development (50 days), some eggs were placed in non-contaminating conditions. During the experiment, the uptake and depuration kinetics, and distribution of these metals among the egg compartments (i.e. eggshell, vitellus, peri-vitelline fluid and embryo) were assessed. In parallel, experiments were conducted with sub-lethal concentrations of stable Ag and Cd (2 and 1 mu g l(-1), respectively) to compare the metal behaviour at higher concentrations. From the spawning date up to I month of development, both metals were taken up efficiently by the eggs, reaching load/concentration ratio (LCR) of 1059... |
| Tipo: Text |
Palavras-chave: Cephalopod; Permeability; Eggshell; Embryo; Biokinetics; Silver; Cadmium. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3882.pdf |
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| Oliveira,Maria Emilia Franco; Cordeiro,Mabel Freitas; Ferreira,Roberta Machado; Souza,Samuel Figueirêdo; Pieroni,Juliana Souza Pinto; Rodrigues,Luiz Fernando de Souza; Fonseca,Jeferson Ferreira da; Vicente,Wilter Ricardo Russiano. |
| The objective was to evaluate if supplemental LH given at the end of FSH treatment would synchronize the time of ovulation and increase the ovulation rate and embryo yield in Santa Ines ewes. Twenty superovulatory (SOV) programs were accomplished in cross-over design (60d interval). On D0, a CIDR device was inserted, and the device was replaced with a new one 7 days later, when 37.5µg of d-cloprostenol was administered. On D12, we started the SOV treatment, administering 256mg of pFSH 8 times, 12h apart. On D14, the CIDR was removed, and 200IU of eCG and 37.5µg of d-cloprostenol were administered. On D15, the ewes were allocated into one of two groups, a Control group (n=10) that received no supplemental LH and a LH group (n=10) treated with 7.5mg of LH... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Superovulation; LH; Ovulation; Embryo; Sheep. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600021 |
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| Gualhanone,A; Furlan,RL; Fernandez-Alarcon,MF; Macari,M. |
| Two experiments were carried out to study the effect of breeder age on incubation parameters (hatchability, eggshell thickness, egg surface temperature and chick weight). In Exp. 1, fertile eggs (30- and 60-wk-old breeders) were incubated at three different temperatures (36.8, 37.8 and 38.8 ºC). Eggshell surface temperature was measured by attaching a thermocouple to the shell and data were collected in a datalogger every ten minutes. This study was conducted according to a 3 x 2 factorial design (three temperatures and two breeder ages). Data revealed that eggshell surface temperature changed according to incubation temperature, with the main increase occurring between 10 and 13 days of incubation, and that the maximum increase in eggshell surface... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler breeder; Development; Embryo; Temperature; Incubation. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000100002 |
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| Maiorka,A; Silva,AVF; Santin,E; Bruno,LDG; Boleli,IC; Macari,M. |
| ABSTRACT This study investigated the effect of broiler breeder age on the morphological development of the small intestine broiler embryos (villus height, crypt depth, microvillus height, and villus density) at 20 day of incubation. Eggs obtained from 30- and 60-wk-old broiler breeders were used. The results showed that embryos from older broiler breeders presented longer villi in the duodenum, jejunum, and ileum compared with younger broiler breeders. In addition, embryos from older broiler breeders presented deeper crypts in the jejunum and ileum, longer microvilli in jejunal enterocytes, and lower villus density (microvillus number/mm2) in the duodenum and ileum than younger breeders. These results suggest that breeder age influences the gut mucosa... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler breeder age; Embryo; Microvillus; Small intestine; Villus. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600079 |
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| Araújo,ICS; Leandro,NSM; Mesquita,MA; Café,MB; Mello,HHC; Gonzales,E. |
| ABSTRACT Two experiments were conducted to evaluate the effect of broiler breeder age and incubator type on hatching parameters, hatch window, embryo diagnosis results, and hatchling physical quality. The treatments consisted of a combination of three broiler breeder ages (29, 35 and 59 weeks of age) and two incubator types (single stage, SS; or and multiple stage, MS). A completely randomized design in a 3x2 factorial arrangement was applied. In Experiment I, 1,896 eggs were used and 360 eggs in Experiment II. There was an interaction between breeder age and incubator type only for hatchling physical quality score. Independently of incubator type, hatchability rate, late embryo mortality, and egg contamination were higher in the eggs laid by older... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Hatch window; Multiple-stage and single-stage incubation. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000600017 |
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| Edagha,Innocent A; Shokunbi,Matthew T; Adenipekun,Adeniyi A; Bassey,Rosemary B; Ekong,Moses B. |
| The aim was to study the effect of preconception gamma irradiation on the gross morphometry of the adult female mice and its embryo. Twenty-seven mice; 18 females and 9 males: subdivided into 3 groups namely (Control, Non-Irradiation and Radiation) containing 6 females and 3 male mice each in 2:1 ratio. A gamma irradiation dose of 1Gy/min was delivered to each batch of mice exposed by a Cobalt 60, Theratron 780c model, by Atomic Energy of Canada Limited (AECL) at the Radiotherapy department of the University College Hospital, Ibadan. All the animals were mated 1 week post irradiation. Vaginal plugs were confirmed, and the pregnant females were sacrificed on day 14 of gestation by chloroform inhalation. The gross morphology of the female mice and their... |
| Tipo: Journal article |
Palavras-chave: Preconception; Irradiation; Gamma ray; Spinal cord; Embryo. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000200039 |
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| Registros recuperados: 67 | |
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